Publications

Andreas D. Flouris, Leonidas G. Ioannou & Lars Nybo (2022) Working in a warming world: Translating thermal physiology to policy-relevant information, Temperature, 9:3, 223-226, DOI: 10.1080/23328940.2022.2086414 Abstract: Temperature and the affiliated scientific community are a leading forum for discussion and dissemination of scientific evidence relevant to the societal challenges of global warming. In an Editorial published at the onset of the HEAT-SHIELD project, which is an inter-sectoral research project funded by the European Commission, we advocated for thermal physiologists to lead the research required to fill knowledge gaps on the issue of occupational heat stress [1]. We vowed to collaborate with researchers all over the world and exchange knowledge on how to integrate scientific evidence into effective, sustainable, and feasible actions that may be adopted by workers, employers, and policy makers. Five years later, we are delighted to see that the scientific community has responded to this call for action and collaboration, and that the occupational heat stress literature has...

Vliora M., Nintou E., Karligiotou E., Ioannou L.G., Grillo E., Mitola S., Flouris A.D. Implication of Irisin in Different Types of Cancer: A Systematic Review and Meta-Analysis. Int. J. Mol. Sci. 2022, 23, 9971. https://doi.org/10.3390/ijms23179971 Abstract: Cancer is a set of diseases characterized by several hallmark properties, such as increased angiogenesis, proliferation, invasion, and metastasis. The increased angiogenic activity constantly supplies the tumors with nutrients and a plethora of cytokines to ensure cell survival. Along these cytokines is a newly discovered protein, called irisin, which is released into the circulation after physical exercise. Irisin is the product of fibronectin type III domain-containing protein 5 (FNDC5) proteolytic cleavage. Recently it has been the topic of investigation in several types of cancer. In this study, we conducted a systematic review and meta-analysis to investigate its implication in different types of cancer. Our results suggest that irisin expression is decreased in cancer patients, thus it can be used as...

Ioannou L.G., Mantzios K., Tsoutsoubi L., Notley S.R., Dinas P.C., Brearley M., Epstein Y., Havenith G., Sawka M.N., Bröde P., Mekjavic I.B., Kenny G.P., Bernard T.E., Nybo L. & Flouris A.D. (2022) Indicators to assess physiological heat strain – Part 1: Systematic review, Temperature, DOI: 10.1080/23328940.2022.2037376. Abstract: In a series of three companion papers published in this Journal, we identify and validate the available thermal stress indicators (TSIs). In this first paper of the series, we conducted a systematic review (registration: INPLASY202090088) to identify all TSIs and provide reliable information regarding their use (funded by EU Horizon 2020; HEAT-SHIELD). Eight databases (PubMed, Agricultural and Environmental Science Collection, Web of Science, Scopus, Embase, Russian Science Citation Index, MEDLINE, and Google Scholar) were searched from database inception to 15 April 2020. No restrictions on language or study design were applied. Of the 879 publications identified, 232 records were considered for further analysis. This search identified 340 instruments and indicators developed...

Vliora M, Ravelli C, Grillo E, Corsini M, Flouris AD, Mitola S. The impact of adipokines on vascular networks in adipose tissue. Cytokine Growth Factor Rev. 2022 Jul 23:S1359-6101(22)00057-0. doi: 10.1016/j.cytogfr.2022.07.008. Epub ahead of print. PMID: 35953434. Abstract: Adipose tissue (AT) is a highly active and plastic endocrine organ. It secretes numerous soluble molecules known as adipokines, which act locally to AT control the remodel and homeostasis or exert pleiotropic functions in different peripheral organs. Aberrant production or loss of certain adipokines contributes to AT dysfunction associated with metabolic disorders, including obesity. The AT plasticity is strictly related to tissue vascularization. Angiogenesis supports the AT expansion, while regression of blood vessels is associated with AT hypoxia, which in turn mediates tissue inflammation, fibrosis and metabolic dysfunction. Several adipokines can regulate endothelial cell functions and are endowed with either pro- or anti-angiogenic properties. Here we address the role of adipokines in the regulation of...

Tsoutsoubi L, Ioannou LG, Mantzios K, Ziaka S, Nybo L and Flouris AD. (2022) Cardiovascular Stress and Characteristics of Cold-Induced Vasodilation in Women and Men during Cold-Water Immersion: A Randomized Control Study. MDPI Journals, Biology 2022, 11(7), 1054; https://doi.org/10.3390/biology11071054. Abstract: Background: Cold-induced vasodilation (CIVD) is a phenomenon that refers to a paradoxical increase in finger temperature that sometimes occurs during cold exposure. The aim of this study was to compare CIVD responses between women and men, during exposure to different environmental conditions. Methods: Seven men and seven women participated in a matched controlled study consisting of a familiarization protocol followed by three experimental sessions (cool (10.8 °C WBGT), thermoneutral (17.2 °C WBGT), and hot (27.2 °C WBGT)). In each session, participants were asked to immerse their left hand and foot in warm water (35 ± 1 °C) for five minutes. Thereafter, the left hand and foot were immersed in cold water (8 ± 1 °C) for 40 min. After...

Dinas PC, Nintou E, Vliora M, Pravednikova AE, Sakellariou P, Witkowicz A, Kachaev ZM, Kerchev VV, Larina SN, Cotton J, Kowalska A, Gkiata P, Bargiota A, Khachatryan ZA, Hovhannisyan AA, Antonosyan MA, Margaryan S, Partyka A, Bogdanski P, Szulinska M, Kregielska-Narozna M, Czepczyński R, Ruchała M, Tomkiewicz A, Yepiskoposyan L, Karabon L, Shidlovskii Y, Metsios GS, Flouris AD. Prevalence of uncoupling protein one genetic polymorphisms and their relationship with cardiovascular and metabolic health. PLoS One. 2022 Apr 28;17(4):e0266386. doi: 10.1371/journal.pone.0266386. PMID: 35482655; PMCID: PMC9049362. Abstract: Contribution of UCP1 single nucleotide polymorphisms (SNPs) to susceptibility for cardiometabolic pathologies (CMP) and their involvement in specific risk factors for these conditions varies across populations. We tested whether UCP1 SNPs A-3826G, A-1766G, Ala64Thr and A-112C are associated with common CMP and their risk factors across Armenia, Greece, Poland, Russia and United Kingdom. This case-control study included genotyping of these SNPs, from 2,283 Caucasians. Results were extended via systematic review and meta-analysis....

Ioannou LG, Foster J, Morris NB, Piil JF, Havenith G, Mekjavic IB, Kenny GP, Nybo L, & Flouris AD,  (2022). Occupational heat strain in outdoor workers: A comprehensive review and meta-analysis. Temperature, DOI: 10.1080/23328940.2022.2030634 Abstract: The present comprehensive review (i) summarizes the current knowledge on the impacts of occupational heat stress on outdoor workers, (ii) provides a historical background on this issue, (iii) presents a meta-analysis of published data, (iv) explores inter-individual and intra-individual factors, (v) discusses the available heat mitigation strategies, (vi) estimates physical work capacity, labour productivity, and metabolic rate for the year 2030, and (vii) provides an overview of existing policy and legal frameworks on occupational heat exposure. Meta-analytic findings from 38 field studies that involved monitoring 2,409 outdoor workers across 41 jobs in 21 countries suggest that occupational heat stress increases the core (r = 0.44) and skin (r = 0.44) temperatures, as well as the heart rate (r =...

Kapnia AK, Dallas CN, Gerodimos V, Flouris AD. Impact of Warm-Up on Muscle Temperature and Athletic Performance. Research Quarterly for Exercise and Sport 2022. DOI: 10.1080/02701367.2021.2007212. Abstract: Purpose: We performed two studies to investigate: the minute-by-minute changes in muscle temperature following a 20-min warm-up routine (Study-1) and the impact of the typical post-warm-up period of inactivity on the performance of basketball athletes (Study-2). Method: In Study-1, 26 males (age: 23.6 ± 6.2 yr; BMI: 24.1 ± 3.1 kg/m2) performed a 20-min cycling warm-up and then rested for 20 min. Tibialis anterior muscle temperature was assessed throughout. In Study-2, six male professional basketball players (age: 24.9 ± 4.6 yr; BMI: 25.5 ± 1.8 kg/m2) performed a series of basketball performance tests after a 20-min warm-up, as well as 9-min and 23-min into a post-warm-up period of inactivity. Results: On average, muscle temperature increased by 0.1°C every minute during warm-up and dropped by the...

Vliora M, Grillo E, Corsini M, Ravelli C, Nintou E, Karligiotou E, Flouris AD, Mitola S. Irisin regulates thermogenesis and lipolysis in 3T3-L1 adipocytes. Biochim Biophys Acta Gen Subj. 2022 Apr;1866(4):130085. doi: 10.1016/j.bbagen.2022.130085. Epub 2022 Jan 10. PMID: 35016977. Abstract: Background: Adipose tissue plays a pivotal role in the development and progression of the metabolic syndrome which along with its complications is an epidemic of the 21st century. Irisin is an adipo-myokine secreted mainly by skeletal muscle and targeting, among others, adipose tissue. In brown adipose tissue it upregulates uncoupling protein-1 (UCP1) which is responsible for mitochondrial non-shivering thermogenesis. Methods: Here we analyzed the effects of irisin on the metabolic activity of 3T3-L1 derived adipocytes through a mitochondrial flux assay. We also assessed the effects of irisin on the intracellular signaling through Western Blot. Finally, the gene expression of ucp1 and lipolytic genes was examined through RT-qPCR. Results: Irisin affects mitochondrial respiration and...

Ioannou LG, Tsoutsoubi L, Mantzios K, Vliora M, Nintou E, Piil JF, Notley SR, Dinas PC, Gourzoulidis GA, Havenith G, Brearley M, Mekjavic IB, Kenny GP, Nybo L, and Flouris AD. Indicators to assess physiological heat strain – Part 3: Multi-country field evaluation and consensus recommendations. Temperature 2022. https://doi.org/10.1080/23328940.2022.2044739. Abstract: In a series of three companion papers published in this Journal, we identify and validate the available thermal stress indicators (TSIs). In this third paper, we conducted field experiments across nine countries to evaluate the efficacy of 61 meteorology-based TSIs for assessing the physiological strain experienced by individuals working in the heat. We monitored 372 experi-enced and acclimatized workers during 893 full work shifts. We continuously assessed core body temperature, mean skin temperature, and heart rate data together with pre/post urine specific gravity and color. The TSIs were evaluated against 17 published criteria covering physiological parameters, practicality, cost effectiveness, and health guidance...